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Anaerobic biochemist

Neoplants · Saint-Ouen-sur-Seine · publiée le 31 août 2026 · ouverte depuis 48 jours · Salaire non communiqué

Les missions

Neoplants is seeking a highly skilled and motivated scientist to join our team focused on engineering biological nitrogen fixation pathways into plants and bacteria as well as optimizing nitrogenase activity. This role is pivotal for driving innovation in synthetic biology, protein expression / purification, and biochemical characterization of complex enzyme systems, particularly the nitrogenase enzyme. Objective: Lead and execute research projects focused on the expression, purification, characterization, and optimization of nitrogenase and associated components in heterologous systems (E. coli) and for subsequent deployment in plant systems. [...]
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Annonce complèteAbout the Role Neoplants is seeking a highly skilled and motivated scientist to join our team focused on engineering biological nitrogen fixation pathways into plants and bacteria as well as optimizing nitrogenase activity. This role is pivotal for driving innovation in synthetic biology, protein expression / purification, and biochemical characterization of complex enzyme systems, particularly the nitrogenase enzyme. Objective: Lead and execute research projects focused on the expression, purification, characterization, and optimization of nitrogenase and associated components in heterologous systems (E. coli) and for subsequent deployment in plant systems. This includes developing and performing robust anaerobic biochemical assays. Working at Neoplants, is a unique opportunity to work on cutting-edge research and impactful projects that can literally change the world alongside a collaborative and interdisciplinary highly engaged team. Main mission (but not limited to): - Design and optimize genetic constructs for heterologous expression of nitrogenase components and accessory proteins in microbial hosts (E. coli). - Develop and implement protocols for strict anaerobic growth and manipulation of relevant microbial strains. - Establish, optimize, and perform in vitro anaerobic activity assays (ammonia production) for nitrogenase components. - Purify and characterize nitrogenase proteins and accessory factors, ensuring structural integrity and functionality under anaerobic conditions. - Assess nitrogenase activity and expression in plant systems and engineered microbial co-cultures. - Document experimental protocols, results, and data analyses in compliance with GLP, biosafety, and intellectual property guidelines. - Become the go to person for setting anaerobic techniques and biochemical assays. - Contribute to cross-functional projects by providing technical expertise in protein biochemistry and anaerobic enzymology. Key Techniques: - Strict anaerobic microbiology techniques and manipulation ( glove box, anaerobic chambers). - High-yield protein expression (e.g., E. coli) and multi-step protein purification (Akta system). - Development and execution of quantitative anaerobic enzyme activity assays (Ammonia detection). - Detailed biochemical and biophysical characterization of purified proteins (SDS-PAGE, Western blot, spectroscopy). - Genetic editing tools (CRISPR/Cas) for microbial strain engineering. - Yeast mitochondrial transformation and recombinant protein expression. Job requirements: - Ph.D. or M.Sc . in Biochemistry, Microbiology, Synthetic Biology, or related discipline with a focus on enzymology. - 3 to 5+ years of hands-on experience working with oxygen-sensitive enzymes, specifically Nitrogenase. - Extensive experience with strict anaerobic microbial growth and handling techniques. - Proven expertise in high-yield recombinant protein expression and multi-step purification from microbial systems. - Deep expertise in protein biochemistry, enzyme kinetics, and assay development under anaerobic conditions. - Experience in assessing nitrogenase activity / expression in planta or complex biological systems is highly desirable. - Ability to work autonomously with little or no supervision - Proactive and action-driven mindset - Proficiency in English Shared values : Team spirit, positive impact, excellence, humility We are looking for passionate and talented experts to help us lay the foundations of what will become the next range of biotechnology products. Why join us? 🌿 Gain a unique opportunity to work on cutting-edge plant biotechnology projects with real-world impact and contribute to global innovation by collaborating with world-class experts in their field 🌿 Grow through permanent learnings in a a very collaborative environment with training opportunities and learn from the founder through regular updates on everything that happens in an innovative start-up 🌿 Participate in fostering team moments, such as our annual kick-off, offsite seminar, after-work, celebrations every month, team activities (e.g. escape games, climbing, sailing or even surfing) 🌿 Come work in our brand new Neolab, ‘The Seed’: A unique 1,200m2 space on two floors featuring a state-of-the-art wetlab, full-scale performance testing rooms among other high-end equipment, as well as open offices and great team spaces! Location: greater Paris area Starting date: ASAP Recruitment process: 1️⃣ HR introductory “get-to-know” interview (video call) 2️⃣ Technical interview focusing on role-related knowledge (video call) 3️⃣ Technical interview focusing on general cognitive ability (case study – in person or video call) 4️⃣ Leadership and cultural fit interview (in person) 5️⃣ Culture fit interview, Meet the team & visit the Seed (in person) Does this sound like the right opportunity for you? Join Neoplants and help us cultivate a greener, healthier future for everyone. We receive a lot of applications, so don’t be shy - surprise us ;) Neoplants values diversity within its organization and in its relationships with its stakeholders. Therefore, when candidates have equal skills, we review all applications without discrimination.

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